A wound is a break in the continuity of the skin. The body’s response to injury and the restoration of the same is healing. Wound healing is a biological process that is initiated by trauma and often terminated by scar formation. In this research, guava leaf extract was used as antiseptic and its efficacy was tested to commercially available products. The result revealed that all mice which received guava leaf extract formed scar earlier as compared to povidone iodine and PNSS. Since maturation phase which also refers to remodeling phase is responsible for the new epithelium and final scar tissue formation, and as the development of these completes the complex process of wound healing, the researchers suggest that the use of guava leaf extract in comparison with povidone iodine and PNSS when it comes to wound healing is the most effective treatment in promoting normal and more rapid wound healing.