This study aimed to investigate the immunological, hematological and biochemical effects of low and high doses of aspirin in male albino rats and to clarify the modulatory influence of vitamin C on the negative impacts of aspirin. Thirty five (35) adult male albino rats (140-160 g) have been split into five groups. The first group (G1) was deemed a control group, wherein 1 ml/kg of saline solution was given to rats. A low dose of aspirin (0.2 mg/kg) alone was administered orally to the second group (G2). A high dose of aspirin (0.6 mg/kg) was administered orally to the third group (G3). The forth group (G4) received oral treatment with a low dose of aspirin and vitamin C (200 mg/Kg). A high dose of aspirin, in combination with vitamin C (200 mg/kg), was given to the fifth group (G5). The findings obtained at the end of the experimental duration (four weeks) showed that the low and high doses of aspirin caused significant rises in serum IgG, monocytes count, RBCs count, blood Hb, PCV%, serum creatinine, AST and ALT and significant decreases in serum IgM, neutrophils count, lymphocytes count and AFT. The low dose of aspirin did not induce significant changes in MDA, SOD, HDL-C, TC and TP levels, While, aspirin in the high dose only induced significant increases in TC and MDA levels, and, significant decreases in SOD, HDL-C and TP levels. Moreover, aspirin at both doses did not alter the total WBCs count, serum IgE, serum IgA, platelets count and LDL-C levels relative to the control group. Vitamin C administration along with aspirin reduced the immunological, hematological and biochemical alterations induced by aspirin. Conclusion: Aspirin in either low or high doses induced disturbances in both humoral and cell-mediated immunity and alterations in hematological parameters and caused adverse effects in liver and kidney functions. Moreover, the two doses of aspirin did not exhibit carcinogenic or anemic effects and the side effects of aspirin were dose-dependent as the low dose had less adverse effects than the high dose. Vitamin C supplements exhibited immunomodulatory effects and could counteract the toxic effects of aspirin intake.