Objectives: Ear is prone to various kinds of infections out of which chronic otitis media is the most common type. Squamosal disease of chronic otitis media is associated with cholesteatoma and is prone to secondary infections especially by aerobic bacteria. This study was done to assess culture and sensitivity of preoperative aural swab and swab from purulent material from mastoid with cholesteatoma obtained intraoperatively during surgery. Comparative study has been made