INTRODUCTION – Diabetic foot ulcers are a common complication in patients suffering from diabetes. Most of the diabetic foot ulcers get infected which leads to ischemia, gangrene and necrosis of the limb, requiring its amputation. Thus, accurate identification of micro-organism infecting the diabetic foot ulcers is needed for their better management. Methods – A prospective study was undertaken with 100 diabetic foot ulcer patients who presented to surgery OPD between 2018 and 2020. Specimens were collected from the ulcer sites at the time of admission after thoroughly washing the site with sterile normal saline and debridement. RESULTS – Overall, the most common organism isolated from the diabetic foot ulcers were Staphylococcus aureus (38%) followed by Pseudomonas spp. (21%) and E. coli (13%). Overall, Piperacillin-Tazobactum (PIT) was found to be the most sensitive (76%) drug towards the organisms isolated followed by Netilmicin (73%), Levofloxacin (68%). CONCLUSION – Staphylococcus aureus is the most common organism to be isolated from the DFU’s and are mostly sensitive to Linezolid and Vancomycin. PiperacillinTazobactum (PIT) showed highest sensitivity of 76% towards all the bacterial isolates which can be used empirically in management of diabetic foot ulcers.