Background: The mouse mammary tumor virus (MMTV)-like env gene have previously been reported to be present in some human breast cancers, however, their role in developing that cancers is still unclear. Aim: This study aims to investigate the presence of MMTV-like env gene among Iraqi women who have breast cancers. Methods: The prevalence of MMTV-like env gene and beta-globulin gene in formalin-fixed paraffin-embedded (FFPE) breast tissue specimens of 88 Iraqi women with breast cancers was detected using conventional PCR and Real- Time techniques. Eight benign breast tumors’ specimens were also used as controls. Fisher’s exact test was used to analyse data. Results: MMTV-like env gene were detected in 16 (18.8%) specimens of those with breast malignancies, while they were not identified in benign breast tumors specimens. However, no significant difference between two groups was noted. Conclusion: MMTV-like env and beta-globin genes were detected in Iraqi women with breast cancer, and further research is needed to study the role and association of these env gene with cancer development.