Abstract:Background: S.aureus is an increasing cause of infections in both immunities and hospitals. It is also a frequent colonizer of the skin and nose. The incidence of hospital-acquired and community-acquired S.aureus infection has risen associated with the simultaneous increase of drug-resistant strains called methicillin resistance S.aureus.Aims and objectives: To determine S. aureus and MRSA's nasal carriage rate among healthcare workers of a teaching hospital, detect the mecA gene, MIC of Vancomycin, and Mupirocin in MRSA isolates.Materials and methods: A total of 285 health care workers from a teaching hospital of coastal Karnataka has participated in this study. Both anterior nares were swabbed and processed in the microbiology laboratory. Standard bacteriological methods identified isolates. PCR confirmed MRSA strains. Results: A total of 285 healthcare workers in a teaching hospital were screened for nasal carriage of S.aureus. Out of 285, 45 (15.8 %) healthcare workers were screened positive for S.aureus. Fifteen (5.3%) were Methicillin-Resistant Staphylococcus Aureus (MRSA). All the MRSA strains were confirmed by mecA gene detection by PCR.Conclusion: The rate of prevalence of MRSA carriage (5.3°/c) in healthcare workers was relatively low. There was no vancomycin-resistant S.umum.t, and MIC creep to vancomycin to MRSA was not observed. Two mupirocin- resistant .S. aureus and significant numbers of MethicillinResistant Coagulase Negative Staphylococcus (MRCoNS) were identified. These findings highlight the urgency for application to infection control measures that aim to decrease M RSA transmission.