A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of dolasetron in capsule dosage form. Waters Spherisorb® 4.6x250mm i.d; column of 5µm particle size, with mobile phase consisting 0.01M ammonium acetate in water :methanol :tetra hydrofuran in the ratio 700:240:60 was used. The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retentiontime was 11.57 min. The detector response was linear in the concentration of 20-60µg/ml. The respective linear regression equation being Y=3000181x+356238.2. The limit of detection and limit of quantification was 0.5µg/ml and 0.15µg/ml respectively. The assay of dolasetron in bulk was found to be 99.85%. From the recovery studies it was found that about 191.10 % on average of dolasetron was recovered which indicates high accuracy of the method. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate,which is useful for the routine determination of dolasetron in bulk drug and in its pharmaceutical dosage form.