Kadamb (Neolamarkiacadamba Roxb.) tree has wide spectrum of bioactivities such as analgesic, antipyretic, anti-inflammatory hypolipidemic and antidiabetic. However, very little is known about anti-microbial properties of its fruits. Therefore, the present study was undertaken to investigate Phytochemical constitution, antibacterial and antifungal properties of N. cadambafruits. Methanol and ethylacetate Extracts of the fruits are prepared and were used to investigate Phytochemical constituents and antimicrobial properties.Out of the selected solvent extracts, the methanolic extract(1000μg/ml) showed maximum zone of inhibition (19.3 mm) against Bacillus subtilis and minimum (14.9 mm) against Enterobacter aerogenes. Ethylacetate extract showed maximum zone of inhibition against Staphylococcus aureus(16.7mm) and minimum against Pseudomonas aeruginosa(11mm). The preliminary phytochemical analysis showed the presence of phytosterols, tannin, phenol, saponins and flavonoids in the methanolic extract. The antimicrobial effects of Neolamarckiacadamba fruit extracts indicate that this wild cinchona contains substantialnumber of bioactive agents are responsible for antimicrobial efficacy. The study also concludes that methanolic extract of N. cadambafruit can be used as a potential antimicrobial source for various infections.