To evaluate pharmacognostic features including macroscopic, microscopic and physicochemical parameters of the five plants Argyreia speciosa, Balanitesa egyptiaca, Gloriosa superb, Tagetes erecta & Cyathocline purpurea. Micro and Macroscopic features of fresh and dried root samples were explored. Physicochemical variables was done by implementing WHO urged variables, preliminary phytochemical and fluorescent evaluation of root sample had been performed intended for correct recognition and standardization of All five plants Argyreia speciosa, Balanitesa egyptiaca, Gloriosa superb, Tagetes erecta & Cyathocline purpurea. The colour, shape, size, odor, and surface features had been observed from the root and then powder root material of all five plants. Light electron microscope i.e. Olympus CX-21i trinocular Microscope images of cross section of root and powdered root revealed that the existence of cork cells, Xylem fibers with tapered ends, lignified xylem vessels, phloem fibers, medullary rays, sclerides and parenchymatous cells. Phytochemical testing revealed the existence of flavonoids, alkaloids, tannins, phenols, steroids, acid compounds, glycosides, amino acids, and proteins. Physicochemical parameters including moisture content, ash value, extractive value and fluorescent behavior of root powder had been identified. These variables are useful which will distinguish the powdered drug material. The present studies useful in an attempt to augment the data with regards to its standardization, identity and performing additional exploration on Ayurveda approach to medicine.