ABSTRACT Background: Hepatitis B virus and hepatitis C virus are two of the most commonly transmitted infectious agents by Blood transmitting so it is still remains a considerable global health problem, this prospective cross-sectional study was conducted between 2015 and 2019 at the directorate of main Blood Bank, dialysis center, thalassemia center and public health laboratory in Thi-Qar Province -Iraq, during that period a total of 1323 patients, 948 (71.7%) males and 375 (28.3%) females, they suffering from signs and symptoms of liver diseases. The aim of study: There were no adequate epidemiological studies on the prevalence of viral hepatitis, especially in last years and there were no national solutions to limit it’s, we conduct this study to determine the prevalence of both types at Thi-qar province. Sampling and methods: This study was conducted in January 2015 to December 2019, on main blood bank, patients referred to the thalassemic center, renal dialysis unit and public health laboratory at Thi-Qar province, the study was conducted on 176848 individuals, from those (1323) cases, (515) blood donors, (45) thalassemic patients, (91) renal failure patients and public health laboratory (672), the following data were recorded: Age, sex, date, resident, type of infection, data were collected by from statistical units. Data were analyzed using SPSS for windows (version 23.0, SPSS lnc, Chicago, III), for comparison between groups as appropriate; P ≤ 0.01 was considered statistically significant. The results: The results of infection were found 1323(0.7%), its distributed as :672 (50.8%) were public health laboratory patients, 515 (38.9%) blood donors, 91 (6.9%) renal dialysis patients and 45(3.4%) thalassemic patients, the results was showed higher infection in males more than females, with high prevalence in age groups (31- 40) and (41-50) years, with significant difference (P<0.01) . Conclusions: despite the findings revealed that Thi-Qar is acceptable percentage rate of prevalence of infection, but may be increased the infection by Immigration from endemic areas, especially for business , transfusion of blood, a major roots of infections by renal dialysis, thalassemic patients were a risk groups because administration of blood.