Aim: To Study on Gender Determination Using Mesiodistal Dimensions of Anterior Teeth. Methods: A prospective observational study was conducted in the Department of Dentistry, Jawaharlal Nehru Medical College and Hospital, Bhagalpur, Bihar, India, ffrom1 year. Total 70 study subjects that were randomly were included in this study. The age of the patients was between 20-30 years with no history or clinical evidence of crown restoration, orthodontic treatment, trauma. After obtaining informed consent, Mesiodistal dimensions of maxillary anterior teeth were measured between anatomical contact points with vernier calliper held parallel to occlusal plane. Results: The results of the study showed that 13 and 23 showedsexual dimorphism whereas other teeth 11-22 didnot show any statistically significant sexual dimorphism. When the level of accuracy for sex determination wasmeasured using 13 separately for males and females it wasfound that 45 % females were classified correctly and55% males were classified correctly. When the level of accuracy for sex determination wasmeasured using 23 separately for males and females it wasfound that 62% females were classified correctly and 62% males were classified correctly. Conclusion: The study showed maxillary canines exhibiting significant sexual dimorphism and can be used for sex determination along with other procedures.