Anti-thyroid peroxides antibody and Thyroglobulin antibody is a member of thyroid autoantibodies which are important in inducing and also diagnosing autoimmune thyroid diseases. Thyroid autoimmunity can cause several thyroid disorders including Graves’s disease, Hashimoto thyroiditis, atrophic autoimmune thyroiditis, hypothyroidism, postpartum thyroiditis, and thyroidassociated ophthalmopathy. Hashimoto thyroiditis and Graves disease are the two most common types, sharing many features immunologically. Anti-thyroid peroxidase antibody and Thyroglobulin antibody is important in diagnosing autoimmune thyroid diseases and judging treatment efficacy. To evaluate the relationship between serum levels of anti-TPO antibody and Thyroglobulin antibody with thyroid function test parameters (T3, T4, and TSH) in hypothyroidism patients. The levels of T3 (Mean - 157.81andStandard Deviation - 43.99), T4 (Mean - 12.39, and Standard Deviation - 3.43) and TSH (Mean -5.52and Standard Deviation - 2.66) are raised anti-TPO antibody and Thyroglobulin antibody titers. A correlation between TSH and T4 levels and abnormal anti-TPO antibody andThyroglobulin antibodywas detected (P <0.000) in hypothyroidism patients. Our results confirm the correlation between thyroid function test with anti-TPO antibodyand Thyroglobulin antibodyvalues, indicating suggested that, a through clinical examination and follow up of individuals with high anti-TPO antibody and Thyroglobulin antibodytiter.