Background: Dengue is an acute viral disease, which includes Dengue fever (DF), dengue hemorrhagic fever (DHF) and Dengue shock syndrome (DSS). The disease has expanded over the last few decades causing high morbidity and mortality. Aim of the study: The study was aimed to determine the seroprevalence of dengue infection among patients attending in Zydus Medical College and Hospital, Dahod, Gujarat during the period from January 2018 to December 2020. Methods: A total of 5252 serum samples from the suspected cases of dengue were processed in Microbiology Laboratory for detecting dengue NS1 antigen by ELISA test. Results: Out of 5252 samples tested, 840 were positive for NS1 antigen by ELISA test contributing case positivity rate of 15.99%. The disease was more prevalent in adult age group of 21- 30 years (29.60%) and was more common in male (65.20%) than female (34.80%). We also reported the seasonal trend of dengue in our district where the case positivity rate was more in monsoon and post monsoon season. Conclusion: It is evident from the present study, that the laboratory test confirming dengue should be extensively used for the purpose of surveillance and control.