ntroduction: The hepatitis B virus is the most common and important of the hepatitis viruses. HBV and HCV are transmitted through a variety of ways, including parenteral, sexual, and vertical transmission (Perinatal). Around 1.4 million people die each year as a result of viral hepatitis; HBV and HCV account for around 90% of these deaths, while other hepatitis viruses account for 10%. Despite the fact that viral hepatitis is a serious public health issue around the world, it has not been given the attention it deserves until now. Recently, the World Health Organization's "2030 Agenda for Sustainable Development Goals" outlined particular steps to avoid viral hepatitis. Aim & Objectives: • To find out the seroprevalence of HBsAg and HCV in the Dahod area. • To find the co-infection between HBsAg and HCV. • To find out the pathogenic potential of HBsAg / HCV and Co-infections in rural populations Materials and Method: Hospital-based cross-sectional study was conducted at Zydus Medical College and Hospital, Dahod, Gujarat, during the period of Jan 2020 – Dec 2020.The study group of patients was clinically diagnosed and admitted in various units such as Intensive care units (ICU) and health care units (wards). According to normal standards, 20,200 blood samples were collected and processed for HBsAg and 6250 blood samples were obtained and processed for HCV antibody testing using immunochromatographic (ICT) methods Result: On hospital-based cross-sectional analysis the year 2020, seroprevalence of HbsAg was 3.72% and HCV 0.54% which was lower in comparison to other parts of India where usually it has been reported. But, here none of the HbsAg and HCV co-infection detected. Conclusion: The tribal area of the Dahod district shows a low seroprevalence of HBsAg and HCV. The availability of recent estimates of HBV, HCV, and associated co-infections, seroprevalence is required to evaluate control efforts and health-care planning, according to this study.