Calcium is an integral component of the skeleton. It acts as a reservoir of calcium for all essential activities of the body. Menopause can initiate osteoporosis through elevated bone remodeling, which occurs predominantly in postmenopausal women. There seems to be an increased risk of fractures in post-menopausal women due to estrogen reduction. Thus, the present study was undertaken to observe the serum calcium and alkaline phosphatase (ALP) levels in post-menopausal women as these substances are biochemical markers of bone metabolism. In this study, 125 samples were taken out of which 76 were premenopausal and 49 postmenopausal. Serum calcium and serum ALP were measured in the blood samples of both groups. The findings demonstrated that the serum calcium level was significantly lower in the post-menopausal group than in the pre-menopausal group, while the ALP level was slightly higher. Therefore, an increase in bone turnover accelerates bone mass reduction in post-menopausal women, whereas a decrease in bone turnover is associated with the preservation of bone mass.