This abstract illustrate an precise, isocratic RP-HPLC strategy have been created by the creator for the concurrent estimation of artemether and lumefantrine in unadulterated and advertised details by utilizing Inertsil C18 column (250×4.6 mm, 5μ) improved mobile phase containing phosphate buffer(pH 4.5) and acetonitrile in the extent of 40:60 %v/v and Discovery wavelength at 218nm. The retention times were 2.207min and 3.733min for artemether and lumefantrine respectively. The linearity range was found to be 10-30µg/ml for artemether and 20-60µg/ml for lumefantrine individually. The developed method was Validated for specificity, system suitability, precision, linearity, accuracy, Limit of Detection, Limit of Quantification, robustness, and Stability and the examine results acquired for all the approval parameters by this proposed strategy were in reasonable concurrence with ICH standards. Thus, the developed RP-HPLC method represents another good alternative for the already existing HPLC methods especially those using certain types of detectors which are not present in most of the laboratories.