The study was carried out at the end of 2019 and until mid-2020 aimed at identifying the genetic variation between the types of P. americana cockroach isolated from two areas in the city of Samarra, the district of Al-Mutassim and the center of Samarra and the work of comparing it with the international species. Ten samples of adult P. americana cockroaches were isolated DNA also the analysis of the S28 gene to make the sequence of nitrogenous bases and compare with the global species registered in the Global Genebank. The results of the current study showed that the best area for the extract of DNA is the mitochondria from the chest area, and the results of the S28 gene amplification showed that there is a percentage of 99% convergence between the species found in the city of Samarra, and also convergence with the global species registered in the Global Genebank, especially with samples from Iran and the United States of America, by 99%.