Background: Vaginal infection is acknowledged as a significant public health issue that affects women of reproductive age and results in a number of issues. The technique of contraception that is used increases the risk of genital infection. The most frequent cause of vaginal discharge in women of reproductive age is bacterial vaginosis. Although the typical vaginal flora differs from person to person, lactobacilli are often the dominant organism. There are other aerobic and anaerobic species in the bacterial flora. These are regarded as pathogenic in some cases. It is known that pelvic inflammatory illness, preterm premature rupture of the membranes (PROM), post-operative gynecologic infections, and abnormal Papanicolaou smear can all be indicators of bacterial vaginosis. Aim: The goal of the current study was to estimate the prevalence of vaginal infections among women who used various forms of contraception. to determine the bacterial vaginosis prevalence. to discover the best accurate, affordable way to diagnose bacterial vaginosis. Material and Method: The samples of vaginal discharge from women of reproductive age, or between the ages of 20 and 40, were collected and processed as part of the current study, which was a prospective study carried out in the Department of Microbiology. The research included 110 non-pregnant women with vaginal discharge as their primary complaint. All of the study's female participants gave informed permission, and the investigation received institutional ethical approval. Complete data on each patient's age, profession, place of residence, parity, and usage of contraception were gathered. This material also includes a history of odor, dysuria, dyspareunia, itching, and abnormal vaginal discharge. women who were bleeding during the collection of the specimens. Results:Premenopausal women without children who were 20 to 40 years old and used a variety of contraceptive techniques were included in the current study. The study's female participants had an average age of 25.3 ± 2.2 years. Among these women, 126 (61.16%) had tubal ligations following their most recent pregnancies, used condoms, implanted IUCDs, and utilized no form of contraception at all. Both Amsel's and Nugent's Criteria were applied to all of these women in the current investigation. Amsel's Criteria revealed that of 110 nonpregnant women, 70 (65.04%) were positive for BV and 50 (34.95%) were negative (BV). Using Nugent's Criteria, however, revealed that 60 (49.51%) were BV-negative, 10 (8.7%) had BV-in-transition, and 40 (41.74%) had BV. Conclusion:Using Amsel's and Nugent's criteria, the incidence of BV in non-pregnant, reproductive-age women with vaginal discharge was determined to be 60.04% and 40.74%, respectively. This demonstrates that the method of contraception used has an impact on the microbial flora in the vagina, which has significant ramifications for women who have diseases linked to changes in the vaginal ecology, such as BV. Therefore, it is advised that women who use contraception have routine microscopic tests.