Zinc Oxide Nano particles promise an important role in various industries like pharma, fertilizer, textiles and garments due to their specific properties such as anti-fungal, antibacterial and anti-rust. Zinc oxide nanoparticles were prepared by eco-friendly wet chemical method. Formation of ZnO nanoparticles was confirmed by using spectroscopic and microscopic investigations. Samples were characterized using Ultra-Violet Spectroscopy, Fourier Transform Infrared, Scanning Electron Microscopy, Transmission Electron microscope, X-ray Diffraction and four probe Voltage-Current characterizations. Antifungal and antimicrobial activity