A new series of 1,5-disubstituted indole derivatives such as 5-(acetylamino)-1-[(4- flurophenyl)carbonyl]-1H-indole-3-carboxylic acid, 1-[(4-fluorophenyl)carbonyl]-5- [(phenylcarbonyl)amino]-1H-indole-3-carboxylic acid, 1-(4-flurobenzoyl)-5-(4-ethylbenzamido-1Hindole-3-carboxylic acid, 5-(4-nitrobenzamido)-1-(4-flurobenzoyl)-1H-indole-3-carboxylic acid, 5-(4- bromobenzamido)-1-(4-flurobenzoyl)-1H-indole-3-carboxylic acid were synthesized. All the newly synthesized derivatives of 1,5-disubstituted indole derivatives are characterized by spectroscopically and analytically. All compounds were screened for their anti-inflammatory activity. The pharmacological screening of the synthesized compounds showed anti-inflammatory activity ranging from 12.12 to 65.51 % inhibition. The compound I was found to be ne+arly more potent then indomethacin which is used as standard drug. A compound III has shown less activity then indomethacine. Compounds IV and V shown more potent activity than compound –I, II and Indomethacin.