Because of their environmental friendly nature and effectiveness cost wise, green synthesis of nanomaterial’s from plant has become progressively popular. In this research, we used methanol extracts from fresh leaves of Senna alata and Senna hirsuta medicinal plants to synthesize silver (Ag) nanoparticles as bio reducing agents. This method allowed the synthesis of nanoparticles supported by spectrophotometry Ultraviolet-visible, FTIR, SEM, TEM and XRD research. UV-Vis spectra and visual observation showed that after treatment with Ag precursors, the color of the fresh Senna alata and Senna hirsuta leaf extracts turned into light greenish and brownish yellow, respectively. This screened antibacterial activity against five different bacteria, viz. Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli with the use of Disc Diffusion method. Silver nanoparticles extracted from Senna alata and Senna hirsuta leaf extracts, which inhibited B.subtilis (18 & 16), E.faecalis (19 & 14), S.aureus (20 & 14), K.pneumoniae (21& 16) and E.coli (17 and 15), size in mm. The higher Inhibition zone occurs in K.pneumoniae and S.aureus follows. It has been shown that inhibiting bacterial growth, the Methanol extracts of Senna alata leaves containing Ag nanoparticles is comparable with chloramphenicol.