bjectives (Background):The purpose of this study is to categorize the baseline of the sugarregulating hormones in pre-fasting and diabetic humans.Methods:This study has involved 30 normal subjects and 26 diabetic patients for investigating the effect of Ramadan fasting on their glucose regulating hormones. By taking pre-fasting, fasting, and post-fasting samples of the same subjects, the effect of fasting on the glucose regulating hormones was investigated. Results:Significant effects of fasting were observed on all the hormones by some being more affected than the others. Additionally, fasting appears to affect some hormones in different ways between the normal and diabetic subjects. It also appears that the regulation of these hormones is more control in the normal subjects than the diabetics resulting in tight control of the glucose within the normal range during fasting in normal subjects. Conclusion:Compared to the normal, diabetics showed improved regulation of their glucose after fasting. This may indicate that fasting does improve the regulation of glucose and possibly of the glucose regulating hormones.