This experiment was conducted to determine the effect of lyophilized Low Density Lipoproteins (LDL) at 5%, Vitamin E at 0.8% and their mixture in Tris diluent, and its effect on the percentage of individual movement and live sperm and the percentage of total deformities after preservation by cooling and cryopreservation for (48 hours, 1 month and 2 months) in the semen of Holstein bulls, from September 2020 to January 2021. It was in two stages, the first phase of extraction and freezing, from September to October, LDL was extracted from egg yolk and converted into powder and kept in tight cans, as for the second stage, it was in the examination laboratory of the Veterinary Hospital, Al-Muthanna Governorate.Semen was collected from two Holstein bulls, with ages from 2-2.5 years, using an artificial vagina.The sample was divided and distributed to the experimental parameters T2, T3 and T4 using lyophilized LDL and vitamin E and their mixture in semen diluent and compared with the control group (20% egg yolk).The results of the study indicated the superiority of treatment T4 (lyophilized LDL + vitamin E) in the percentage of individual motility and live sperm, a decrease in the percentage of total deformations after preservation by cooling 5 ° C and cryopreservation -196 ° C. Concluded from the foregoing the possibility of using T4 in semen diluent because of its ability to preserve sperm, improve the individual motility rate, and obtain the lowest percentage of deformities during the preservation period.