Online ISSN: 2515-8260

The Effects of Purified Staphylococcal Enterotoxin A on Gene Expression of Nuclear Factor Кappa B (NF-КB) in Rabbits

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Ayat Ali Mahmood1 , Ahmad Darweesh Jabar2 , Muthana Ibrahim Maleek2


ABSTRACT This study aimed to determine the effects of Staphylococcal enterotoxin A on NF-KB gene expression in rabbits. Firstly, SEA was isolate, purified and identified from one strain of Staphylococcus aureus collected from urine sample, and had identified by biochemical and molecular methods. Then ,Staphylococcal enterotoxin A was purified by chromatography technique . All purified fractions of SEA was determined by ELISA kit. Finally, 5mg\ml of SEA was injected intravenously to rabbits in order to demonstrate its effects on NF-KB gene expression. At the end of this experiment, the gene expression of NF-KB increased to 9.1 in compression with control group with 1 of fold time (P = 0.045)

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