The term pit and fissure sealant describes the material that is introduced into the occlusal pits and fissure of teeth, thus forming a protective barrier and prevents the development of caries. The aim of the study was to assess the use of pit and fissure sealants in the adult population. This was a retrospective study and records were assessed from patients visiting dental college from June 2019 to March 2020. Data of pit and fissure sealants performed on patients above the age of 18 years was collected. Excel tabulation was done. SPSS was used to analyze the data and summarize the results. In this present study, pit and fissure sealants were used in 4379 adult patients. Pit and fissure sealant was used more between the age group of 18 to 30 years and most commonly in maxillary first molar. Pit and fissure sealant was used more in maxillary arches with the percentage of 55.2%. Distribution of pit and fissure sealant was used more in male patients with a percentage of 55.2%. From the present study, we can conclude that pit and fissure sealant were used more between the age group of 18 to 20 years. And sealant application was more predominant on maxillary first molar compared to other teeth.