Breast cancer is by far the commonest cancer identified in women internationally, positioned second in both sexes collectively. Breast Self-Examination (BSE) leads to a lesser incidence of progressive breast cancer or mortality from breast cancer among women who were prudently trained in the procedure. Study was done with an objective to evaluate the effectiveness of structured teaching programme on knowledge regarding B.S.E among nursing students. Quantitative approach was considered appropriate with one group pretest & post-test research design. The study was conducted at Faculty of Nursing, SGT University, Budhera, Gurugram, Haryana. The sample were 40 nursing students of age group 18-26years & above who full-fill the inclusion criteria. Non probability Convenience sampling technique was used in the present study. Two Tools were used; First tool consisted of 13 items for obtaining information of demographic variables such as Age, Religion, Education, Type of family etc. Second tool consisted of 25 MCQ’s to evaluate the knowledge regarding breast self-examination amongst nursing students. The ‘t’ value of the mean pre-test knowledge score & post-test knowledge score was found to be -7.37 at level of significant p<0.05. The present study concludes that a structured teaching Program is effective in term of gain in knowledge regarding Breast Self- Examination among nursing students.