Introduction: According to NACO guidelines, all mandatory tests should be carried out on donors blood samples for human immune deficiency virus (HIV), Hepatitis B virus( HBV), Hepatitis C virus (HCV), syphilis and malaria. The whole blood or components from any unit that tests positive should be discarded. Materials and methods: The present study was carried out in H.K.E.S blood bank attached to Mahadevappa Rampure Medical College and Basaveshwar Teaching and General Hospital Gulbarga The blood bank of Mahadevappa Rampure Medical College and Basaveshwar Teaching and General Hospital Gulbarga of Department of Pathology, is licenced blood bank with average annual collection of 10,000 units of blood from healthy blood donors from in and around Gulbarga .